Hotspot

trnC-GCA-petN

LSC · 28175-28880 · 706 bp

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Pi 0.037
MarkerSeek score 76.00
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
706
variable_sites
25
pi
0.037
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCATGCGAGACTTACTA 50.0 50.0 58.1 58.2 736-762 bp 1.0 83.5
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 797-823 bp 1.0 82.1
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG TCTTCCCCATACTACGAGGG 50.0 55.0 58.1 57.6 787-813 bp 1.0 81.9
trnC-GCA-petN_p4 GGGGGACTGCAAATCCTTTT AGCCCATGCGAGACTTACTA 50.0 50.0 58.4 58.2 754-780 bp 1.0 81.2
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTT AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.4 57.6 815-841 bp 1.0 79.8