Hotspot

trnR-UCU-atpA

LSC · 10021-10288 · 268 bp

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Pi 0.105
MarkerSeek score 78.00
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
268
variable_sites
27
pi
0.105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AGCCTTCCAAGCTAACGATG CCTCTACCAAGGCATTCACC 50.0 55.0 58.0 58.0 673-686 bp 1.0 84.4
trnR-UCU-atpA_p2 TTCTGTGAAAGCACCCAGAC CCTCTACCAAGGCATTCACC 50.0 55.0 58.0 58.0 879-892 bp 1.0 83.3
trnR-UCU-atpA_p3 AGCCTTCCAAGCTAACGATG CCCTGGTCGTGAAGCTTATC 50.0 55.0 58.0 58.1 1260-1273 bp 1.0 82.1
trnR-UCU-atpA_p4 AGCCTTCCAAGCTAACGATG CCTGGTCGTGAAGCTTATCC 50.0 55.0 58.0 58.1 1259-1272 bp 1.0 82.1
trnR-UCU-atpA_p5 AGCCTTCCAAGCTAACGATG GGTCGTGAAGCTTATCCAGG 50.0 55.0 58.0 58.1 1256-1269 bp 1.0 82.1