Hotspot

ycf1

SSC · 106381-111741 · 5361 bp

Back to Macrotyloma

Pi 0.00522

MarkerSeek score 48.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5361
variable_sites: 42
pi: 0.00522
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGATGGAAGTCGAATTGCCT	TCAATATCCCCTGTGCTCCA	50.0	50.0	58.0	58.1	1017 bp	1.0	79.7
ycf1_p2	CAGTTTGGGGGATGGAAGTC	TCAATATCCCCTGTGCTCCA	55.0	50.0	58.2	58.1	1026 bp	1.0	78.8
ycf1_p3	AACAGTTTGGGGGATGGAAG	TCAATATCCCCTGTGCTCCA	50.0	50.0	57.7	58.1	1028 bp	1.0	77.6
ycf1_p4	GGGGGATGGAAGTCGAATTG	TCAATATCCCCTGTGCTCCA	55.0	50.0	58.3	58.1	1020 bp	1.0	77.5
ycf1_p5	CAGAAGCCTGGGATAGCATT	TCAATATCCCCTGTGCTCCA	50.0	50.0	57.3	58.1	1392 bp	1.0	74.6