Hotspot

rps3-rps19

IRb · 80436-80974 · 539 bp

Back to Macrotyloma

Pi 0.00495

MarkerSeek score 60.60

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: IRb
length_bp: 539
variable_sites: 4
pi: 0.00495
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CAAGTCTGAAACCAAGCGGA	TAAACTTCCGAGGACATGCG	50.0	50.0	58.4	58.0	623-635 bp	1.0	77.2
rps3-rps19_p2	CAAGTCTGAAACCAAGCGGA	TCCGAGGACATGCGAAAAAT	50.0	45.0	58.4	57.9	617-629 bp	1.0	76.4
rps3-rps19_p3	CAAGTCTGAAACCAAGCGGA	TTCCGAGGACATGCGAAAAA	50.0	45.0	58.4	58.1	618-630 bp	1.0	76.3
rps3-rps19_p4	AAGTCTGAAACCAAGCGGAT	TAAACTTCCGAGGACATGCG	45.0	50.0	57.4	58.0	622-634 bp	1.0	75.9
rps3-rps19_p5	AGTCTGAAACCAAGCGGATT	TAAACTTCCGAGGACATGCG	45.0	50.0	57.4	58.0	621-633 bp	1.0	75.9