Hotspot

rbcL-trnK-UUU

LSC · 72379-73254 · 876 bp

Back to Macrotyloma

Pi 0.011

MarkerSeek score 55.10

Primer available yes

Label rbcL-trnK-UUU

Feature Metadata

label_name: rbcL-trnK-UUU
feature_type: igs
region: LSC
length_bp: 876
variable_sites: 13
pi: 0.011
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-trnK-UUU_p1	GCTTGCGAAGTATGGAAGGA	AGAGTCGCTCTTAAAAGCCG	50.0	50.0	58.0	58.0	969-991 bp	1.0	83.5
rbcL-trnK-UUU_p2	GCTTGCGAAGTATGGAAGGA	AGTCGCTCTTAAAAGCCGAG	50.0	50.0	58.0	58.0	967-989 bp	1.0	83.5
rbcL-trnK-UUU_p3	GCTTGCGAAGTATGGAAGGA	GAGTCGCTCTTAAAAGCCGA	50.0	50.0	58.0	58.0	968-990 bp	1.0	83.4
rbcL-trnK-UUU_p4	GCTTGCGAAGTATGGAAGGA	AGCTTTTCCCACATCAAGCA	50.0	45.0	58.0	58.0	1320-1343 bp	1.0	82.6
rbcL-trnK-UUU_p5	AGTCCTGAATTAGCTGCTGC	AGCTTTTCCCACATCAAGCA	50.0	45.0	58.0	58.0	1338-1361 bp	1.0	82.5