Hotspot

trnT-GGU-psbD

LSC · 48251-49422 · 1172 bp

Back to Macrotyloma

Pi 0.00739

MarkerSeek score 61.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1172
variable_sites: 13
pi: 0.00739
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CCGCAACCAGTCATCCATAA	55.0	50.0	58.1	58.0	1302-1304 bp	1.0	80.0
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	ATAGACCAGACCAACCCACA	55.0	50.0	58.1	58.0	1339-1341 bp	1.0	80.0
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	CCGCAACCAGTCATCCATAA	50.0	50.0	58.1	58.0	1305-1307 bp	1.0	79.8
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ATAGACCAGACCAACCCACA	50.0	50.0	58.1	58.0	1342-1344 bp	1.0	79.8
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	AGACCAACCCACAAAAACGA	55.0	45.0	58.1	57.9	1332-1334 bp	1.0	79.2