Hotspot

rpoB-trnC-GCA

LSC · 43439-44433 · 995 bp

Back to Macrotyloma

Pi 0.00603

MarkerSeek score 61.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 995
variable_sites: 9
pi: 0.00603
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTTCTGTTAAGCCCCGATC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.0	58.1	1158-1167 bp	1.0	80.2
rpoB-trnC-GCA_p2	AAAGCCCTTCTGTTAAGCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1163-1172 bp	1.0	80.0
rpoB-trnC-GCA_p3	CCTTCTGTTAAGCCCCGATC	CTTATCGATCAGGCGACACC	55.0	55.0	58.0	58.2	1177-1186 bp	1.0	79.0
rpoB-trnC-GCA_p4	AAAGCCCTTCTGTTAAGCCC	CTTATCGATCAGGCGACACC	50.0	55.0	58.1	58.2	1182-1191 bp	1.0	78.8
rpoB-trnC-GCA_p5	CCTTCTGTTAAGCCCCGATC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.0	58.4	1140-1149 bp	1.0	78.1