Hotspot

psbJ-petA

LSC · 16540-17331 · 792 bp

Back to Macrotyloma

Pi 0.00842

MarkerSeek score 63.90

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 10
pi: 0.00842
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	CATATTCCGGATTGGGCTCA	CGTGTCGAAGGTCTCTTGTT	50.0	50.0	57.4	57.9	931-940 bp	1.0	75.3
psbJ-petA_p2	ATTCCGGATTGGGCTCATCT	CGTGTCGAAGGTCTCTTGTT	50.0	50.0	58.9	57.9	928-937 bp	1.0	73.0
psbJ-petA_p3	TATTCCGGATTGGGCTCATC	CGTGTCGAAGGTCTCTTGTT	50.0	50.0	57.1	57.9	929-938 bp	1.0	73.0
psbJ-petA_p4	TCATATTCCGGATTGGGCTC	CGTGTCGAAGGTCTCTTGTT	50.0	50.0	57.1	57.9	932-941 bp	1.0	73.0
psbJ-petA_p5	CATATTCCGGATTGGGCTCAT	CGTGTCGAAGGTCTCTTGTT	47.6	50.0	57.9	57.9	931-940 bp	1.0	71.2