Hotspot

petL-psbE

LSC · 14822-15753 · 932 bp

Back to Macrotyloma

Pi 0.00501

MarkerSeek score 62.60

Primer available yes

Label petL-psbE

Feature Metadata

label_name: petL-psbE
feature_type: igs
region: LSC
length_bp: 932
variable_sites: 7
pi: 0.00501
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-psbE_p1	AGACCAATAAATAGAGACGAGGT	AGAACGTTCTCCTGTGCTTC	39.1	50.0	56.5	57.8	1033-1039 bp	1.0	44.0
petL-psbE_p2	AGACCAATAAATAGAGACGAGGT	GTTCTCCTGTGCTTCCAGAC	39.1	55.0	56.5	58.2	1028-1034 bp	1.0	43.7
petL-psbE_p3	AGACCAATAAATAGAGACGAGGT	CCTGCAATGAATAGGGAGGG	39.1	55.0	56.5	57.7	1107-1113 bp	1.0	42.9
petL-psbE_p4	AGACCAATAAATAGAGACGAGGT	TCTCCTGTGCTTCCAGACAT	39.1	50.0	56.5	58.4	1026-1032 bp	1.0	42.5
petL-psbE_p5	AGACCAATAAATAGAGACGAGGTT	AGAACGTTCTCCTGTGCTTC	37.5	50.0	57.2	57.8	1033-1039 bp	1.0	41.3