Hotspot

psaC-ycf1

SSC · 98257-98817 · 561 bp

Back to Macrosolen

Pi 0.171

MarkerSeek score 75.70

Primer available yes

Label psaC-ycf1

Feature Metadata

label_name: psaC-ycf1
feature_type: igs
region: SSC
length_bp: 561
variable_sites: 149
pi: 0.171
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ycf1_p1	CACATTGAGTGCACCCGATA	GAACCGATTCGTCTCTCTGT	50.0	50.0	58.0	57.1	1052-1152 bp	0.75	72.3
psaC-ycf1_p2	CATTGAGTGCACCCGATACA	GAACCGATTCGTCTCTCTGT	50.0	50.0	58.0	57.1	1050-1150 bp	0.75	72.3
psaC-ycf1_p3	TTGAGTGCACCCGATACATG	GAACCGATTCGTCTCTCTGT	50.0	50.0	58.0	57.1	1048-1148 bp	0.75	72.3
psaC-ycf1_p4	ACATTGAGTGCACCCGATAC	GAACCGATTCGTCTCTCTGT	50.0	50.0	58.0	57.1	1051-1151 bp	0.75	72.3
psaC-ycf1_p5	GACACAGTCCTCCGTTCTTG	GAACCGATTCGTCTCTCTGT	55.0	50.0	58.2	57.1	1147-1247 bp	0.75	70.4