Hotspot

psbE-petL

LSC · 52177-53132 · 956 bp

Back to Macrosolen

Pi 0.0758
MarkerSeek score 83.70
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
956
variable_sites
134
pi
0.0758
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 GTCCGTGAATAGCCAACCAT ATCAAAGGTCCAACCGATCG 50.0 50.0 58.0 58.0 1616-1675 bp 0.75 81.5
psbE-petL_p2 TCGGATTAGGTTCAGCGTTG ATCAAAGGTCCAACCGATCG 50.0 50.0 58.0 58.0 1723-1748 bp 0.75 74.9
psbE-petL_p3 TGCATTGCTGATATGGACCC ATCAAAGGTCCAACCGATCG 50.0 50.0 58.0 58.0 1696-1721 bp 0.75 74.9
psbE-petL_p4 TCGGATTAGGTTCAGCGTTG AGAACGTGGGTCTCCAAAAC 50.0 50.0 58.0 58.0 1909-1932 bp 0.75 74.5
psbE-petL_p5 TGCATTGCTGATATGGACCC AGAACGTGGGTCTCCAAAAC 50.0 50.0 58.0 58.0 1882-1905 bp 0.75 74.5