Hotspot

trnT-GGU-psbD

LSC · 26991-27664 · 674 bp

Back to Macrosolen

Pi 0.0902

MarkerSeek score 89.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 674
variable_sites: 117
pi: 0.0902
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 89.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	TCGTAACCAGTCATCCATACT	50.0	42.9	58.3	55.9	781-1153 bp	1.0	64.9
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	CTTCGTAACCAGTCATCCAT	50.0	45.0	58.3	54.9	783-1155 bp	1.0	64.7
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	CTTCGTAACCAGTCATCCATACT	50.0	43.5	58.3	57.7	783-1155 bp	1.0	63.6
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	TTCGTAACCAGTCATCCATACT	50.0	40.9	58.3	56.6	782-1154 bp	1.0	62.9
trnT-GGU-psbD_p5	CTCAGTGGTAGAGTAACGCC	TCGTAACCAGTCATCCATACT	55.0	42.9	57.4	55.9	808-1180 bp	1.0	62.7