Hotspot

trnE-UUC-trnT-GGU

LSC · 26433-26918 · 486 bp

Back to Macrosolen

Pi 0.095

MarkerSeek score 81.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 486
variable_sites: 84
pi: 0.095
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGGATTTGAACCAGCGTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	721-864 bp	1.0	91.9
trnE-UUC-trnT-GGU_p2	GGCATCGACCCAGAAAGATT	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	686-801 bp	0.75	74.4
trnE-UUC-trnT-GGU_p3	CTTTTGTAGTGCCCTACCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.9	58.0	628-743 bp	0.75	74.1
trnE-UUC-trnT-GGU_p4	CGGATTTGAACCAGCGTAGA	CGTCCGAGGATTTGACCATT	50.0	50.0	58.0	58.0	1376-1863 bp	0.75	73.8
trnE-UUC-trnT-GGU_p5	GGCATCGACCCAGAAAGATT	CGTCCGAGGATTTGACCATT	50.0	50.0	58.0	58.0	1313-1800 bp	0.75	73.6