Hotspot

ycf3

LSC · 44057-46131 · 2075 bp

Back to Macrolenes

Pi 0.0179

MarkerSeek score 56.40

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2075
variable_sites: 37
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	CATTTGACTAATCAGACTAATAAATCA	TCCTTTGTTTGTGGGGAATCA	25.9	42.9	54.2	57.7	2175-2184 bp	1.0	41.8
ycf3_p2	CATTTGACTAATCAGACTAATAAATC	TCCTTTGTTTGTGGGGAATCA	26.9	42.9	52.7	57.7	2175-2184 bp	1.0	41.8
ycf3_p3	CATTTGACTAATCAGACTAATAAATCA	ACAATTCCTTTGTTTGTGGGG	25.9	42.9	54.2	57.2	2180-2189 bp	1.0	41.8
ycf3_p4	CATTTGACTAATCAGACTAATAAATCA	CCTTTGTTTGTGGGGAATCA	25.9	45.0	54.2	56.1	2174-2183 bp	1.0	41.8
ycf3_p5	CATTTGACTAATCAGACTAATAAATCA	CAATTCCTTTGTTTGTGGGGA	25.9	42.9	54.2	56.9	2179-2188 bp	1.0	41.8