Hotspot

trnI-CAU-rpl23

IRa · 154252-154434 · 183 bp

Back to Macrolenes

Pi 0.0118
MarkerSeek score 62.80
Primer available yes
Label trnI-CAU-rpl23

Feature Metadata

label_name
trnI-CAU-rpl23
feature_type
igs
region
IRa
length_bp
183
variable_sites
2
pi
0.0118
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-rpl23_p1 GAATGGTTAAAGCGCCCAAC TCGACCCAATGCTTTACTTCT 50.0 42.9 58.0 57.3 350-364 bp 1.0 69.9
trnI-CAU-rpl23_p2 GAATGGTTAAAGCGCCCAAC CGACCCAATGCTTTACTTCTG 50.0 47.6 58.0 57.3 349-363 bp 1.0 69.5
trnI-CAU-rpl23_p3 GAATGGTTAAAGCGCCCAAC TTCGACCCAATGCTTTACTTCT 50.0 40.9 58.0 58.0 351-365 bp 1.0 67.4
trnI-CAU-rpl23_p4 GCTAAGCATCCATGGCTGAA TCGACCCAATGCTTTACTTCT 50.0 42.9 58.3 57.3 367-381 bp 1.0 67.3
trnI-CAU-rpl23_p5 GCTAAGCATCCATGGCTGAA CGACCCAATGCTTTACTTCTG 50.0 47.6 58.3 57.3 366-380 bp 1.0 66.9