Hotspot

ndhD-psaC

SSC · 119143-119282 · 140 bp

Back to Macrolenes

Pi 0.0429

MarkerSeek score 77.10

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 140
variable_sites: 6
pi: 0.0429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGAAAATTAAAGAACCCGCCA	ATGGCATGAAACCACTCGAA	38.1	45.0	56.0	57.8	247-249 bp	1.0	56.7
ndhD-psaC_p2	AGAAAATTAAAGAACCCGCCA	CACTCGAAGCATGGGTCTAG	38.1	55.0	56.0	57.8	235-237 bp	1.0	56.7
ndhD-psaC_p3	AGAAAATTAAAGAACCCGCCA	AGAGATGTGAATCCGCTTGC	38.1	50.0	56.0	58.3	299-301 bp	1.0	55.3
ndhD-psaC_p4	AAGAAAATTAAAGAACCCGCCA	ATGGCATGAAACCACTCGAA	36.4	45.0	56.8	57.8	248-250 bp	1.0	54.6
ndhD-psaC_p5	AGAAAATTAAAGAACCCGCCAA	ATGGCATGAAACCACTCGAA	36.4	45.0	56.8	57.8	247-249 bp	1.0	54.6