Hotspot

trnC-GCA-ndhJ

LSC · 49797-50642 · 846 bp

Back to Macrolenes

Pi 0.00721
MarkerSeek score 64.50
Primer available yes
Label trnC-GCA-ndhJ

Feature Metadata

label_name
trnC-GCA-ndhJ
feature_type
igs
region
LSC
length_bp
846
variable_sites
6
pi
0.00721
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA ACTCATCCACGACTGAAACG 55.0 50.0 58.0 57.9 1010-1024 bp 1.0 81.0
trnC-GCA-ndhJ_p2 GGGATAGCTCAGCTGGTAGA CTCATCCACGACTGAAACGT 55.0 50.0 58.0 57.9 1009-1023 bp 1.0 81.0
trnC-GCA-ndhJ_p3 GGGATAGCTCAGCTGGTAGA AGTTGGATAGGATGGCCCTT 55.0 50.0 58.0 58.1 974-988 bp 1.0 80.9
trnC-GCA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA ACTCATCCACGACTGAAACG 50.0 50.0 58.1 57.9 974-988 bp 1.0 80.7
trnC-GCA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA CTCATCCACGACTGAAACGT 50.0 50.0 58.1 57.9 973-987 bp 1.0 80.7