Hotspot

rpoB-trnC-GCA

LSC · 27517-28961 · 1445 bp

Back to Macrolenes

Pi 0.0118

MarkerSeek score 56.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1445
variable_sites: 17
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCACTTCCATCCACCAGCAT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	59.0	58.4	1509-1510 bp	1.0	70.1
rpoB-trnC-GCA_p2	TTCCCTCACTTCCATCCACC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	59.0	58.4	1514-1515 bp	1.0	70.0
rpoB-trnC-GCA_p3	GACATTCCCTCACTTCCATCC	AAAAGGATTTGCAGTCCCCC	52.4	50.0	58.1	58.4	1518-1519 bp	1.0	69.5
rpoB-trnC-GCA_p4	CATTCCCTCACTTCCATCCA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	56.9	58.4	1516-1517 bp	1.0	69.2
rpoB-trnC-GCA_p5	ATTCCCTCACTTCCATCCAC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	56.9	58.4	1515-1516 bp	1.0	69.2