Hotspot

trnS-GCU-trnR-UCU

LSC · 8183-10020 · 1838 bp

Back to Macrolenes

Pi 0.0198

MarkerSeek score 70.50

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1838
variable_sites: 36
pi: 0.0198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ACGGAAAGAGAGGGATTCGA	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.2	56.6	1986-2006 bp	1.0	69.5
trnS-GCU-trnR-UCU_p2	TAGCAATCCGACGCTTTAGG	AGAACTTGCGTCCAATAGGA	50.0	45.0	57.8	56.6	1935-1955 bp	1.0	69.0
trnS-GCU-trnR-UCU_p3	ACGGAAAGAGAGGGATTCGA	ACATAGAAGAACTTGCGTCCA	50.0	42.9	58.2	57.3	1993-2013 bp	1.0	67.6
trnS-GCU-trnR-UCU_p4	ACGGAAAGAGAGGGATTCGA	AAGAACTTGCGTCCAATAGGA	50.0	42.9	58.2	57.3	1987-2007 bp	1.0	67.5
trnS-GCU-trnR-UCU_p5	TAGCAATCCGACGCTTTAGG	ACATAGAAGAACTTGCGTCCA	50.0	42.9	57.8	57.3	1942-1962 bp	1.0	67.1