Hotspot

rps16-trnQ-UUG

LSC · 5752-6918 · 1167 bp

Back to Macrolenes

Pi 0.0223
MarkerSeek score 70.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1167
variable_sites
26
pi
0.0223
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAAATCCACGAACGATCCCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.6 58.1 1266-1271 bp 1.0 76.1
rps16-trnQ-UUG_p2 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1232-1237 bp 1.0 74.9
rps16-trnQ-UUG_p3 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 59.1 58.1 1235-1240 bp 1.0 72.7
rps16-trnQ-UUG_p4 AAATCCACGAACGATCCCG GAGGTTCGAATCCTTCCGTC 52.6 55.0 57.9 58.1 1265-1270 bp 1.0 72.7
rps16-trnQ-UUG_p5 AAAATCCACGAACGATCCCG TTCGGAGGTTCGAATCCTTC 50.0 50.0 58.6 57.3 1270-1275 bp 1.0 71.3