Hotspot

tsf

LSC · 9128-9730 · 603 bp

Back to Macrocystis

Pi 0.00332

MarkerSeek score 59.90

Primer available yes

Label tsf

Feature Metadata

label_name: tsf
feature_type: gene
region: LSC
length_bp: 603
variable_sites: 2
pi: 0.00332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tsf_p1	GGGTTCTCACAGTTTCTCCA	AGGTCAAACGTTAATCACAACA	50.0	36.4	57.1	56.5	720 bp	1.0	44.8
tsf_p2	GGGTTCTCACAGTTTCTCCAG	AGGTCAAACGTTAATCACAACA	52.4	36.4	58.2	56.5	720 bp	1.0	42.4
tsf_p3	AGGGTTCTCACAGTTTCTCC	AGGTCAAACGTTAATCACAACA	50.0	36.4	56.8	56.5	721 bp	1.0	42.3
tsf_p4	GGGTTCTCACAGTTTCTCCA	AAGGTCAAACGTTAATCACAACA	50.0	34.8	57.1	57.2	721 bp	1.0	42.2
tsf_p5	GGGTTCTCACAGTTTCTCCA	GGTCAAACGTTAATCACAACA	50.0	38.1	57.1	55.0	719 bp	1.0	40.9