Hotspot

chlL

LSC · 55097-55981 · 885 bp

Back to Macrocystis

Pi 0.00452

MarkerSeek score 55.90

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: LSC
length_bp: 885
variable_sites: 4
pi: 0.00452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	ACCAATCAATATCTCTTAAGAGCGA	TCCCTGTTTCACATTCAAAA	36.0	35.0	57.9	53.2	1100 bp	1.0	40.3
chlL_p2	ACCAATCAATATCTCTTAAGAGCG	TCCCTGTTTCACATTCAAAA	37.5	35.0	56.7	53.2	1100 bp	1.0	40.3
chlL_p3	CCAATCAATATCTCTTAAGAGCGAC	TCCCTGTTTCACATTCAAAA	40.0	35.0	57.7	53.2	1099 bp	1.0	40.3
chlL_p4	ACCAATCAATATCTCTTAAGAGCGA	TCCCTGTTTCACATTCAAAAT	36.0	33.3	57.9	53.9	1100 bp	1.0	40.3
chlL_p5	ACCAATCAATATCTCTTAAGAGCGA	TCCCTGTTTCACATTCAAAATT	36.0	31.8	57.9	54.7	1100 bp	1.0	40.3