Hotspot

chlB

LSC · 21276-22808 · 1533 bp

Back to Macrocystis

Pi 0.00587

MarkerSeek score 50.70

Primer available yes

Label chlB

Feature Metadata

label_name: chlB
feature_type: gene
region: LSC
length_bp: 1533
variable_sites: 9
pi: 0.00587
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB_p1	ACTAGGTTATTTATGTCAAGAAGA	AGAGGGTGTTTCTATTGAACACT	29.2	39.1	53.2	57.4	1629 bp	1.0	40.4
chlB_p2	ACTAGGTTATTTATGTCAAGAAGA	AGGGTGTTTCTATTGAACACTCT	29.2	39.1	53.2	57.4	1627 bp	1.0	40.4
chlB_p3	ACTAGGTTATTTATGTCAAGAAGA	GAGGGTGTTTCTATTGAACACTC	29.2	43.5	53.2	57.1	1628 bp	1.0	40.4
chlB_p4	ACTAGGTTATTTATGTCAAGAAGA	AGGGTGTTTCTATTGAACACTC	29.2	40.9	53.2	56.0	1627 bp	1.0	40.4
chlB_p5	ACTAGGTTATTTATGTCAAGAAGA	GGGTGTTTCTATTGAACACTCT	29.2	40.9	53.2	56.0	1626 bp	1.0	40.4