Hotspot

atpB

LSC · 62470-63915 · 1446 bp

Back to Macrocystis

Pi 0.00346

MarkerSeek score 60.70

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1446
variable_sites: 5
pi: 0.00346
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CTTCGTCGGCAGAAGTATCC	ACTTGTTTTACTTTTGTTCTTAAGCT	55.0	26.9	58.2	56.5	1615 bp	1.0	40.2
atpB_p2	GGTAGAACTACTTCGTCGGC	ACTTGTTTTACTTTTGTTCTTAAGCT	55.0	26.9	57.8	56.5	1625 bp	1.0	40.2
atpB_p3	CTTCGTCGGCAGAAGTATCC	AGACTTGTTTTACTTTTGTTCTTAAGC	55.0	29.6	58.2	57.4	1617 bp	1.0	40.2
atpB_p4	CTTCGTCGGCAGAAGTATCC	GACTTGTTTTACTTTTGTTCTTAAGCT	55.0	29.6	58.2	57.4	1616 bp	1.0	40.2
atpB_p5	GGTAGAACTACTTCGTCGGC	GACTTGTTTTACTTTTGTTCTTAAGCT	55.0	29.6	57.8	57.4	1626 bp	1.0	40.2