Hotspot

psbA-cbbX

LSC · 70188-70885 · 698 bp

Back to Macrocystis

Pi 0.0317

MarkerSeek score 65.80

Primer available yes

Label psbA-cbbX

Feature Metadata

label_name: psbA-cbbX
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 22
pi: 0.0317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-cbbX_p1	CTGCTCCTTCTGTTGTTGGT	GAGCTCGAATGAGACAAGCA	50.0	50.0	58.0	58.0	837-840 bp	1.0	82.2
psbA-cbbX_p2	TGTTGCGATTTCTGCTCCTT	GAGCTCGAATGAGACAAGCA	45.0	50.0	58.1	58.0	848-851 bp	1.0	81.5
psbA-cbbX_p3	TTCCTGTTGCGATTTCTGCT	GAGCTCGAATGAGACAAGCA	45.0	50.0	58.1	58.0	852-855 bp	1.0	81.5
psbA-cbbX_p4	CTTCCTGTTGCGATTTCTGC	GAGCTCGAATGAGACAAGCA	50.0	50.0	57.7	58.0	853-856 bp	1.0	80.3
psbA-cbbX_p5	CTGCTCCTTCTGTTGTTGGT	CGAATGAGACAAGCAAACCG	50.0	50.0	58.0	57.8	832-835 bp	1.0	80.3