Hotspot

chlN-psaF

LSC · 57391-57525 · 135 bp

Back to Macrocystis

Pi 0.0148

MarkerSeek score 61.00

Primer available yes

Label chlN-psaF

Feature Metadata

label_name: chlN-psaF
feature_type: igs
region: LSC
length_bp: 135
variable_sites: 2
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN-psaF_p1	ACCACTTCGCCGTAATCAAA	TGCTGCTAAAGGTATGCTGA	45.0	45.0	57.8	56.9	316 bp	1.0	70.5
chlN-psaF_p2	GACCACTTCGCCGTAATCAA	TGCTGCTAAAGGTATGCTGA	50.0	45.0	58.3	56.9	317 bp	1.0	69.6
chlN-psaF_p3	GCGTTATACTGACCGTCGAT	TGCTGCTAAAGGTATGCTGA	50.0	45.0	57.6	56.9	274 bp	1.0	68.8
chlN-psaF_p4	GAGACCACTTCGCCGTAATC	TGCTGCTAAAGGTATGCTGA	55.0	45.0	58.4	56.9	319 bp	1.0	68.3
chlN-psaF_p5	CAGCGTTATACTGACCGTCG	TGCTGCTAAAGGTATGCTGA	55.0	45.0	58.5	56.9	276 bp	1.0	67.7