Hotspot

psbX-rpl27

LSC · 37632-38061 · 430 bp

Back to Macrocystis

Pi 0.0141

MarkerSeek score 64.20

Primer available yes

Label psbX-rpl27

Feature Metadata

label_name: psbX-rpl27
feature_type: igs
region: LSC
length_bp: 430
variable_sites: 6
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbX-rpl27_p1	ACAATAAGCCCACCAGCAAT	ACCAGGTGATAATGTAGGGGT	45.0	47.6	57.8	57.9	600-604 bp	1.0	70.3
psbX-rpl27_p2	CTACAATAAGCCCACCAGCA	ACCAGGTGATAATGTAGGGGT	50.0	47.6	57.6	57.9	602-606 bp	1.0	68.8
psbX-rpl27_p3	ACTACAATAAGCCCACCAGC	ACCAGGTGATAATGTAGGGGT	50.0	47.6	57.6	57.9	603-607 bp	1.0	68.8
psbX-rpl27_p4	ACAATAAGCCCACCAGCAAT	TGCATTTCAGTATCTGCCACT	45.0	42.9	57.8	57.6	509-513 bp	1.0	68.8
psbX-rpl27_p5	CTACAATAAGCCCACCAGCA	TGCATTTCAGTATCTGCCACT	50.0	42.9	57.6	57.6	511-515 bp	1.0	67.4