Hotspot

trnY-ORF1

LSC · 23102-23144 · 43 bp

Back to Macrocystis

Pi 0.0233

MarkerSeek score 65.00

Primer available yes

Label trnY-ORF1

Feature Metadata

label_name: trnY-ORF1
feature_type: igs
region: LSC
length_bp: 43
variable_sites: 1
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-ORF1_p1	AAGGGGGCGGATTGTAAATC	ACTTAGGTCGAGATGGTCCC	50.0	55.0	57.9	58.2	183 bp	1.0	78.1
trnY-ORF1_p2	AAGGGGGCGGATTGTAAATC	GTCGAGATGGTCCCGATTTT	50.0	50.0	57.9	57.7	177 bp	1.0	77.4
trnY-ORF1_p3	AATCCGCTGGTTTTCCTACG	ACTTAGGTCGAGATGGTCCC	50.0	55.0	58.3	58.2	167 bp	1.0	76.6
trnY-ORF1_p4	AATCCGCTGGTTTTCCTACG	GTCGAGATGGTCCCGATTTT	50.0	50.0	58.3	57.7	161 bp	1.0	75.9
trnY-ORF1_p5	GCTGGTTTTCCTACGTTGGT	ACTTAGGTCGAGATGGTCCC	50.0	55.0	58.4	58.2	162 bp	1.0	75.5