Hotspot

chlI-trnF

LSC · 2830-2944 · 115 bp

Back to Macrocystis

Pi 0.0261

MarkerSeek score 67.50

Primer available yes

Label chlI-trnF

Feature Metadata

label_name: chlI-trnF
feature_type: igs
region: LSC
length_bp: 115
variable_sites: 3
pi: 0.0261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlI-trnF_p1	TGCTTACGTCACCGTCTAAG	GAACTGGTGACACGAGGATC	50.0	55.0	57.4	58.0	267 bp	1.0	75.9
chlI-trnF_p2	TGCTTACGTCACCGTCTAAG	TCCACTGCTCTACCAACTGA	50.0	50.0	57.4	58.0	242 bp	1.0	75.9
chlI-trnF_p3	TGCTTACGTCACCGTCTAAG	TTTGAACTGGTGACACGAGG	50.0	50.0	57.4	58.1	270 bp	1.0	75.5
chlI-trnF_p4	TGCTTACGTCACCGTCTAAG	CTGGTGACACGAGGATCTTC	50.0	55.0	57.4	57.7	264 bp	1.0	73.6
chlI-trnF_p5	GCTTACGTCACCGTCTAAGA	GAACTGGTGACACGAGGATC	50.0	55.0	57.1	58.0	266 bp	1.0	73.6