Hotspot

psaC

SSC · 115576-115821 · 246 bp

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Pi 0.00407

MarkerSeek score 40.40

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 1
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	AAATGGAACCTCCTGCAGTG	CGAAGCATGGTTAGAGCACT	50.0	50.0	58.1	58.0	1446-1454 bp	1.0	80.7
psaC_p2	AAATGGAACCTCCTGCAGTG	AAGCCGAAGCATGGTTAGAG	50.0	50.0	58.1	58.0	1450-1458 bp	1.0	80.6
psaC_p3	AGTACGGGTCTTTGTCGGTA	CGAAGCATGGTTAGAGCACT	50.0	50.0	58.1	58.0	854 bp	0.5	59.2
psaC_p4	TCTCGAGTACGGGTCTTTGT	CGAAGCATGGTTAGAGCACT	50.0	50.0	58.1	58.0	859 bp	0.5	59.1
psaC_p5	AGTACGGGTCTTTGTCGGTA	AAGCCGAAGCATGGTTAGAG	50.0	50.0	58.1	58.0	858 bp	0.5	59.0