Hotspot

ndhD-psaC

SSC · 115447-115575 · 129 bp

Back to Macodes

Pi 0.0465

MarkerSeek score 74.40

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 129
variable_sites: 6
pi: 0.0465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AACCTGCGAATATGGGCAAA	ATGGCATGAAACAACTCGGA	45.0	45.0	58.2	57.8	227-228 bp	1.0	79.3
ndhD-psaC_p2	ACCTGCGAATATGGGCAAAA	ATGGCATGAAACAACTCGGA	45.0	45.0	58.2	57.8	226-227 bp	1.0	79.3
ndhD-psaC_p3	CCTGCGAATATGGGCAAAAC	ATGGCATGAAACAACTCGGA	50.0	45.0	57.8	57.8	225-226 bp	1.0	78.9
ndhD-psaC_p4	AACCTGCGAATATGGGCAAA	AAGAGATGTGAATCCGCCTG	45.0	50.0	58.2	57.7	280-281 bp	1.0	77.9
ndhD-psaC_p5	ACCTGCGAATATGGGCAAAA	AAGAGATGTGAATCCGCCTG	45.0	50.0	58.2	57.7	279-280 bp	1.0	77.9