Hotspot

rpl32-trnL-UAG

SSC · 111633-112513 · 881 bp

Back to Macodes

Pi 0.0299
MarkerSeek score 71.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
881
variable_sites
26
pi
0.0299
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGAAAAAGCTTTTACTGCCGC TCCTAAGAGCAGCGTGTCTA 42.9 50.0 58.0 57.9 1008-1019 bp 1.0 73.8
rpl32-trnL-UAG_p2 GAAAAAGCTTTTACTGCCGCT TCCTAAGAGCAGCGTGTCTA 42.9 50.0 58.0 57.9 1007-1018 bp 1.0 73.8
rpl32-trnL-UAG_p3 AGAAAAAGCTTTTACTGCCGC AGCAGCGTGTCTACCAATTT 42.9 45.0 58.0 57.8 1001-1012 bp 1.0 73.4
rpl32-trnL-UAG_p4 GAAAAAGCTTTTACTGCCGCT AGCAGCGTGTCTACCAATTT 42.9 45.0 58.0 57.8 1000-1011 bp 1.0 73.4
rpl32-trnL-UAG_p5 GAAAAAGCTTTTACTGCCGCT CTAAGAGCAGCGTGTCTACC 42.9 55.0 58.0 57.8 1005-1016 bp 1.0 73.3