Hotspot

ndhF-rpl32

SSC · 111291-111458 · 168 bp

Back to Macodes

Pi 0.037

MarkerSeek score 65.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 168
variable_sites: 6
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCCGAAAACCACATAGCTA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.0	58.0	390-396 bp	1.0	82.1
ndhF-rpl32_p2	ACACATTCCCACAAGTTCCC	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.0	58.0	693-699 bp	1.0	81.4
ndhF-rpl32_p3	CACATTCCCACAAGTTCCCA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.0	58.0	692-698 bp	1.0	81.4
ndhF-rpl32_p4	AAGGGGAAATTGTGCGGATT	TCCACCGGACAGTCAAAAAG	45.0	50.0	58.1	58.0	960-966 bp	1.0	80.7
ndhF-rpl32_p5	AGGGGAAATTGTGCGGATTT	TCCACCGGACAGTCAAAAAG	45.0	50.0	58.1	58.0	959-965 bp	1.0	80.7