Hotspot

rpl14-rpl16

LSC · 79764-79885 · 122 bp

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Pi 0.0168

MarkerSeek score 64.50

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 2
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CCGCTGTTATCCGCTACATT	AAACTGTAGCCAAAGCAGCT	50.0	45.0	58.1	58.0	240-243 bp	1.0	80.5
rpl14-rpl16_p2	CCGCTGTTATCCGCTACATT	GTTAAACCGGGCCGAATACT	50.0	50.0	58.1	58.0	283-286 bp	1.0	80.1
rpl14-rpl16_p3	AATTCTTGAGCTCCGCTGTT	AAACTGTAGCCAAAGCAGCT	45.0	45.0	57.8	58.0	252-255 bp	1.0	79.9
rpl14-rpl16_p4	AATTCTTGAGCTCCGCTGTT	GTTAAACCGGGCCGAATACT	45.0	50.0	57.8	58.0	295-298 bp	1.0	79.6
rpl14-rpl16_p5	TCAATTCTTGAGCTCCGCTG	AAACTGTAGCCAAAGCAGCT	50.0	45.0	58.3	58.0	254-257 bp	1.0	79.3