Hotspot

rpl36-infA

LSC · 78283-78408 · 126 bp

Back to Macodes

Pi 0.0505

MarkerSeek score 67.90

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 126
variable_sites: 5
pi: 0.0505
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	CTTGAGCAGCATAGGGTGTT	GGCGGAATGGGGATTGTAAT	50.0	50.0	57.9	58.0	1051-1077 bp	1.0	81.9
rpl36-infA_p2	CGACCTCGTACGTCTGTAAC	GGCGGAATGGGGATTGTAAT	55.0	50.0	57.8	58.0	977-1003 bp	1.0	81.8
rpl36-infA_p3	GCAGCATAGGGTGTTCCTTT	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.2	58.0	1046-1072 bp	1.0	81.6
rpl36-infA_p4	CTCGTAATGCCGCATCTCTT	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.1	58.0	1153-1179 bp	1.0	81.5
rpl36-infA_p5	TCGTAATGCCGCATCTCTTC	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.1	58.0	1152-1178 bp	1.0	81.5