Hotspot

atpB-rbcL

LSC · 52852-53688 · 837 bp

Back to Macodes

Pi 0.00851

MarkerSeek score 65.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 837
variable_sites: 7
pi: 0.00851
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	967-968 bp	1.0	79.7
atpB-rbcL_p2	CGATACGCCCTAGGTTTTGT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.7	58.1	950-951 bp	1.0	78.4
atpB-rbcL_p3	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	968-969 bp	1.0	77.3
atpB-rbcL_p4	CTGGGCCAATAATTTGAGCG	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.5	58.1	968-969 bp	1.0	76.8
atpB-rbcL_p5	TGGGCCAATAATTTGAGCGA	TCCAACGCTTGCTTTAGTCT	45.0	45.0	57.9	57.5	995-996 bp	1.0	76.2