Hotspot

trnT-GGU-psbD

LSC · 30504-31531 · 1028 bp

Back to Macodes

Pi 0.0182

MarkerSeek score 67.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1028
variable_sites: 18
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1150-1188 bp	1.0	82.6
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1153-1191 bp	1.0	82.4
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1140-1178 bp	1.0	81.5
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CCCTCCGTAACCAATCATCC	55.0	55.0	58.1	57.7	1124-1162 bp	1.0	81.5
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1143-1181 bp	1.0	81.3