Hotspot

trnE-UUC-trnT-GGU

LSC · 30030-30431 · 402 bp

Back to Macodes

Pi 0.0233

MarkerSeek score 65.20

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 402
variable_sites: 9
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	491-506 bp	1.0	82.4
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	470-485 bp	1.0	82.1
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	467-482 bp	1.0	82.0
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	469-484 bp	1.0	81.7
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	TGGAAAAAGCCCCTTATCGG	50.0	50.0	57.8	57.9	515-530 bp	1.0	81.2