Hotspot

rpoB-trnC-GCA

LSC · 25680-26765 · 1086 bp

Back to Macodes

Pi 0.0185

MarkerSeek score 60.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1086
variable_sites: 20
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCTGTCAAGCCCCG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1234-1336 bp	1.0	75.9
rpoB-trnC-GCA_p2	AATTCTTCTGTCAAGCCCCG	CGACACCCAGATTCGAACTG	50.0	55.0	58.2	58.6	1258-1360 bp	1.0	74.9
rpoB-trnC-GCA_p3	AATTCTTCTGTCAAGCCCCG	ATAAAGGATTTGCAGTCCCCC	50.0	47.6	58.2	58.0	1235-1337 bp	1.0	71.8
rpoB-trnC-GCA_p4	CTTCTGTCAAGCCCCGATTA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.3	57.5	1230-1332 bp	1.0	71.8
rpoB-trnC-GCA_p5	CTTCTGTCAAGCCCCGATTA	CGACACCCAGATTCGAACTG	50.0	55.0	57.3	58.6	1254-1356 bp	1.0	70.7