Hotspot

ycf1

SSC · 130805-136477 · 5673 bp

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Pi 0.00441

MarkerSeek score 40.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5673
variable_sites: 25
pi: 0.00441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATGCTATCCCAGGTTTCCG	CAAGCAAACGTACACTCCCT	50.0	50.0	57.7	58.1	234 bp	1.0	78.0
ycf1_p2	AATGCTATCCCAGGTTTCCG	TCAAGCAAACGTACACTCCC	50.0	50.0	57.7	58.1	235 bp	1.0	77.9
ycf1_p3	AATGCTATCCCAGGTTTCCG	AGCAAACGTACACTCCCTTC	50.0	50.0	57.7	57.8	232 bp	1.0	77.6
ycf1_p4	TCCCAGGTTTCCGCTATTTC	CAAGCAAACGTACACTCCCT	50.0	50.0	57.6	58.1	227 bp	1.0	77.5
ycf1_p5	TCCCAGGTTTCCGCTATTTC	TCAAGCAAACGTACACTCCC	50.0	50.0	57.6	58.1	228 bp	1.0	77.4