Hotspot

ndhE-ndhG

SSC · 124770-125048 · 279 bp

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Pi 0.0149

MarkerSeek score 64.80

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 279
variable_sites: 4
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GGCTGGTGATCAAGCCATAG	TTTTAGCCGCTTTGCTAGGT	55.0	45.0	58.4	57.8	384-393 bp	1.0	78.4
ndhE-ndhG_p2	GCTGGTGATCAAGCCATAGA	TTTTAGCCGCTTTGCTAGGT	50.0	45.0	57.4	57.8	383-392 bp	1.0	76.5
ndhE-ndhG_p3	GGCTGGTGATCAAGCCATAG	TTTAGCCGCTTTGCTAGGTG	55.0	50.0	58.4	58.6	383-392 bp	1.0	75.5
ndhE-ndhG_p4	AACCATATTTCGGCTGGTGA	TTTTAGCCGCTTTGCTAGGT	45.0	45.0	57.2	57.8	395-404 bp	1.0	75.1
ndhE-ndhG_p5	ATTTCGGCTGGTGATCAAGC	TTTTAGCCGCTTTGCTAGGT	50.0	45.0	58.9	57.8	389-398 bp	1.0	74.3