Hotspot

ccsA-ndhD

SSC · 122033-122303 · 271 bp

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Pi 0.0332
MarkerSeek score 67.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
271
variable_sites
9
pi
0.0332
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ATTCCGCAATTGTAGCCTCG TCCCGATTTCGTTTTATCACT 50.0 38.1 58.7 55.3 457 bp 1.0 46.6
ccsA-ndhD_p2 CCGCAATTGTAGCCTCGAT TCCCGATTTCGTTTTATCACT 52.6 38.1 58.0 55.3 454 bp 1.0 44.2
ccsA-ndhD_p3 GCAATTGTAGCCTCGATGGG TCCCGATTTCGTTTTATCACT 55.0 38.1 59.1 55.3 452 bp 1.0 43.8
ccsA-ndhD_p4 ATTCCGCAATTGTAGCCTCG TCGATCTCTATTCTTTTACCCGT 50.0 39.1 58.7 56.9 498 bp 1.0 43.0
ccsA-ndhD_p5 ATTCCGCAATTGTAGCCTCG ATCCCGATTTCGTTTTATCACT 50.0 36.4 58.7 55.9 458 bp 1.0 43.0