Hotspot

rpl20-rps12

LSC · 73820-74638 · 819 bp

Back to Maclura

Pi 0.00489

MarkerSeek score 61.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 819
variable_sites: 4
pi: 0.00489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTAAGTCTTGAACGAGCCCC	AAATGTCACGAAATCCCCCG	55.0	50.0	58.3	58.5	980-981 bp	1.0	73.9
rpl20-rps12_p2	TTGGTTCTACGTCTTCGAGC	AAATGTCACGAAATCCCCCG	50.0	50.0	57.7	58.5	932-933 bp	1.0	73.4
rpl20-rps12_p3	TAAGTCTTGAACGAGCCCCT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.4	58.5	979-980 bp	1.0	73.2
rpl20-rps12_p4	GTAAGTCTTGAACGAGCCCC	CAGCGACGAGGAACATGTAC	55.0	55.0	58.3	58.7	943-944 bp	1.0	72.4
rpl20-rps12_p5	TGGTTCTACGTCTTCGAGCT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.5	58.5	931-932 bp	1.0	72.4