Hotspot

ycf4-cemA

LSC · 64605-65543 · 939 bp

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Pi 0.00534

MarkerSeek score 62.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 939
variable_sites: 5
pi: 0.00534
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACTCGTACCG	AGAGAGATCCACCAAGGCAA	55.0	50.0	58.1	58.3	1114-1116 bp	1.0	77.2
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	AGAGAGATCCACCAAGGCAA	45.0	50.0	58.1	58.3	1050-1052 bp	1.0	77.1
ycf4-cemA_p3	ATTGAACAGAAAGCTGCGGA	AGAGAGATCCACCAAGGCAA	45.0	50.0	58.1	58.3	1071-1073 bp	1.0	77.1
ycf4-cemA_p4	TGAACAGAAAGCTGCGGAAT	AGAGAGATCCACCAAGGCAA	45.0	50.0	58.1	58.3	1069-1071 bp	1.0	77.1
ycf4-cemA_p5	TTCCCTTGACTCGTACCGAT	AGAGAGATCCACCAAGGCAA	50.0	50.0	58.2	58.3	1112-1114 bp	1.0	76.6