Hotspot

ndhC-trnV-UAC

LSC · 54832-55265 · 434 bp

Back to Maclura

Pi 0.00935
MarkerSeek score 62.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
434
variable_sites
4
pi
0.00935
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT CGGTTCAAGTCCGTATAGCC 50.0 55.0 57.9 58.2 553-555 bp 1.0 79.1
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT GGTTCAAGTCCGTATAGCCC 50.0 55.0 57.9 57.8 552-554 bp 1.0 78.4
ndhC-trnV-UAC_p3 CTGGTCCTTTGCTAATCGGG CGGTTCAAGTCCGTATAGCC 55.0 55.0 58.3 58.2 570-572 bp 1.0 77.5
ndhC-trnV-UAC_p4 CCTTTGCTAATCGGGGCTAA CGGTTCAAGTCCGTATAGCC 50.0 55.0 57.7 58.2 565-567 bp 1.0 77.5
ndhC-trnV-UAC_p5 CTGGTCCTTTGCTAATCGGG GGTTCAAGTCCGTATAGCCC 55.0 55.0 58.3 57.8 569-571 bp 1.0 76.9