Hotspot

trnF-GAA-ndhJ

LSC · 52413-53124 · 712 bp

Back to Maclura

Pi 0.00562

MarkerSeek score 62.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 4
pi: 0.00562
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GAAAGCTGGATAGGATGGCC	55.0	55.0	58.0	58.1	857-861 bp	1.0	79.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GAAAGCTGGATAGGATGGCC	50.0	55.0	58.1	58.1	821-825 bp	1.0	79.2
trnF-GAA-ndhJ_p3	TGATGCGTAAGGAATGGTCG	GAAAGCTGGATAGGATGGCC	50.0	55.0	58.1	58.1	876-880 bp	1.0	79.1
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	GAAAGCTGGATAGGATGGCC	55.0	55.0	57.9	58.1	845-849 bp	1.0	78.5
trnF-GAA-ndhJ_p5	AAGGAATGGTCGGGATAGCT	GAAAGCTGGATAGGATGGCC	50.0	55.0	58.2	58.1	868-872 bp	1.0	78.1