Hotspot

rps4-trnT-UGU

LSC · 49513-49998 · 486 bp

Back to Maclura

Pi 0.00426

MarkerSeek score 62.00

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 486
variable_sites: 2
pi: 0.00426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CGGGGCGTAATTGGTTTCTA	TTGTAATGCGAGGGTCATCG	50.0	50.0	58.0	58.1	627-642 bp	1.0	81.5
rps4-trnT-UGU_p2	CCCCCAGACGACGTATTTTT	TTGTAATGCGAGGGTCATCG	50.0	50.0	57.9	58.1	561-576 bp	1.0	81.2
rps4-trnT-UGU_p3	CGGGGCGTAATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	649-664 bp	1.0	80.9
rps4-trnT-UGU_p4	CCCCCAGACGACGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	583-598 bp	1.0	80.5
rps4-trnT-UGU_p5	CTAGTTAGGCCCGGTAAAGC	TTGTAATGCGAGGGTCATCG	55.0	50.0	57.8	58.1	580-595 bp	1.0	80.0