Hotspot

rps16-trnQ-UUG

LSC · 6620-7332 · 713 bp

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Pi 0.0112
MarkerSeek score 58.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
713
variable_sites
8
pi
0.0112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 793-799 bp 1.0 80.3
rps16-trnQ-UUG_p2 ACGTTGCCTTCTACCACATC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 779-785 bp 1.0 79.6
rps16-trnQ-UUG_p3 GTTGCCTTCTACCACATCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 777-783 bp 1.0 79.6
rps16-trnQ-UUG_p4 ATCGTGTCTTTCAAGTCGCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.9 58.1 798-804 bp 1.0 79.1
rps16-trnQ-UUG_p5 CACGTTGCCTTCTACCACAT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 780-786 bp 1.0 78.8