Hotspot

trnH-GUG-psbA

LSC · 91-483 · 393 bp

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Pi 0.0138

MarkerSeek score 57.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 393
variable_sites: 5
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	437-466 bp	1.0	83.3
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	440-469 bp	1.0	83.2
trnH-GUG-psbA_p3	TAATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.1	57.9	491-520 bp	1.0	83.1
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	438-467 bp	1.0	83.1
trnH-GUG-psbA_p5	TAATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.1	57.9	494-523 bp	1.0	83.0