Hotspot

ndhF

SSC · 116189-118567 · 2379 bp

Back to Macleaya

Pi 0.00758

MarkerSeek score 56.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2379
variable_sites: 18
pi: 0.00758
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCCACCTAGTTTGTCAGCTT	TGACCAATTGTAACTTCTTGA	45.0	33.3	56.4	53.0	2476-2567 bp	1.0	40.7
ndhF_p2	TCCACCTAGTTTGTCAGCTT	TTGACCAATTGTAACTTCTTGA	45.0	31.8	56.4	53.9	2477-2568 bp	1.0	40.7
ndhF_p3	TCCACCTAGTTTGTCAGCTT	TGATTTGATCATTTGACCAATTGT	45.0	29.2	56.4	55.8	2489-2580 bp	1.0	40.7
ndhF_p4	TCCACCTAGTTTGTCAGCTT	TCATTTGACCAATTGTAACTTCTTGA	45.0	30.8	56.4	57.8	2481-2572 bp	1.0	40.7
ndhF_p5	TCCACCTAGTTTGTCAGCTT	TGACCAATTGTAACTTCTTGATTTGA	45.0	30.8	56.4	57.8	2476-2567 bp	1.0	40.7